Biochemical hydrogen demand (BOD
) is an environmental monitoring indicator, mainly used to monitor the pollution of organic matter in water. Generally, organic matter can be decomposed by microorganisms, but microorganisms need to consume oxygen when decomposing organic compounds in water. Dissolved oxygen is not enough to supply the needs of microorganisms, and the water body is in a state of pollution. Biochemical oxygen demand (BOD), as an important indicator in water quality environmental monitoring, its measurement method has been continuously improved and developed. At present, the dilution and inoculation method is widely used in water quality monitoring, comparative experiments, and arbitration analysis as a classic method, and the microbial sensor method is used as a rapid determination method in rapid determination and analysis. In addition, there are pressure measurement, temperature increase, and activity Sludge aeration degradation method, pressure test coulomb method, platform value method, etc. are used in specific environments or research analysis. Dilution and inoculation method Dilution and inoculation method means that the water sample is moderately diluted or inoculated and cultured at 20°C for 5 days to measure the mass concentration of dissolved oxygen in the water sample before and after cultivation. The amount of dissolved oxygen consumed by a liter of sample, expressed in the form of BOD5. Its national standard (H505-2009) was revised by the Ministry of Environmental Protection in 2009. Microbial sensor rapid determination method The principle of microbial sensor rapid determination method is that when the water sample containing saturated dissolved oxygen enters the flow cell and contacts the microbial sensor, the soluble and biodegradable organic matter in the water sample is affected by the bacteria in the microbial film, so that The mass of oxygen diffused onto the surface of the oxygen electrode is reduced. When the diffusion rate (mass) of the biodegradable organic matter in the water sample to the bacterial film reaches a constant value, the mass of oxygen diffused to the surface of the oxygen electrode also reaches a constant value at this time, thus producing a constant current due to the constant current and water sample There is a quantitative relationship between the difference in the concentration of biodegradable organic matter in the water and the amount of oxygen reduction, based on which the biochemical oxygen demand in the water sample can be converted.' In 2002, China promulgated the microbial sensor rapid determination method (HJ/T86-2002 ). The method is widely used in surface water, domestic sewage, and industrial wastewater, but the measurement process is complicated and takes 5 days. The timeliness is poor; the microbial sensor method is fast, convenient, and timeliness. Real-time grasp of this water pollution index greatly improves work efficiency and is widely used in environmental monitoring stations, colleges and universities, urban sewage treatment and various surface water and industrial wastewater.
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