The Necessary Of Dissolved Oxygen Measurement

Dissolved oxygen refers to the amount of oxygen that has dissolved into a water source, which can be anything from the ocean to a bioreactor. Whether your company handles the transportation of live fish or is involved in wastewater treatment, it is critical to measure and track the amount of dissolved oxygen in your water.

The necessity of dissolved oxygen measurement depends on the application you are using it for. If your company is involved in aquaculture in some way, low dissolved oxygen levels will cause fish to suffocate. As for sewage treatment, it is necessary to break down solid waste. Lower dissolved oxygen levels cause death, meaning the breakdown of waste products stops. No matter what industrial or aquaculture application you are involved in, the measurement of dissolved oxygen is very important. Being able to respond quickly to changes in dissolved oxygen levels can save a lot of time and money.

How does a dissolved oxygen meter work?

When performing fermentations and cell cultures, dissolved oxygen meters are critical for measuring dissolved oxygen in bioreactors and cell cultures. Whether you are developing new drugs or studying the biochemistry of cells, the correct amount of dissolved oxygen is critical for a bioreactor to work efficiently. Low dissolved oxygen levels in bioreactors can cause problems with cell growth rates and nutrient uptake, meaning your research and experiments may fail entirely.

To avoid these problems, you need to continuously measure dissolved oxygen levels in your bioreactor or cell culture environment, which requires the use of a dissolved oxygen sensor. As long as these sensors are installed correctly and kept away from air bubbles that could cause false readings, they should give you correct measurements. By installing these probes in a bioreactor or cell culture environment, you will be able to maintain the dissolved oxygen levels required for your cell culture or fermentation process.

What do dissolved oxygen levels mean?

Although most fermentation and cell culture applications require high dissolved oxygen levels, too high a dissolved oxygen level can cause harmful cellular mutations. Whether you use a current DO sensor or an optical DO sensor, you can get a dissolved oxygen measurement in as little as 1-3 minutes. If you don't use one of these probes, you won't be able to determine the amount of oxygen dissolved in the water, which means you won't get accurate results. Regardless of your reason for using fermentation or cell culture, proper and accurate results require proper dissolved oxygen levels.

Effects of Dissolved Oxygen Concentration on Cell Culture Growth and Production

The concentration of dissolved oxygen is very important to the growth and production of cell cultures more than almost any other application. When dissolved oxygen levels get too high or drop too low, a number of adverse effects can occur. When the dissolved oxygen content in the bioreactor is too low, the growth rate will slow down, the absorption of nutrients will deteriorate, and the synthesis of metabolites will be affected, which will lead to a decrease in the quality of the final product and a reduction in yield.

On the other hand, higher dissolved oxygen levels can lead to the formation of reactive oxygen species, which are highly unstable molecules that can cause cell death. Some components of the substance may also become oxidized, causing cellular mutations. If you happen to be using a large bioreactor equipped with a compressor for air injection, high dissolved oxygen levels will also cause you to waste energy. Whether the dissolved oxygen level is too low or too high, you will not get the expected results from your ongoing cell culture.

For all the previously mentioned adverse effects, it is important to measure the amount of dissolved oxygen in the bioreactor. If dissolved oxygen levels are less than ideal, you will experience slow growth rates, the production of unstable reactive oxygen species, or wasted energy using larger bioreactors, all of which will make your results less accurate. Although higher dissolved oxygen concentrations do lead to increased growth and productivity in cell cultures, the production of large amounts of reactive oxygen species ultimately impairs cell growth and exacerbates cell mutations. Dissolved oxygen measurement is crucial if you want your cell culture process to be fully optimized and efficient.

Care and maintenance of dissolved oxygen meter

If you plan to use your dissolved oxygen meter continuously, you must care for the sensor and maintain it properly. Well-maintained sensors last longer than poorly maintained sensors, ensuring you make a sound investment. First, it is recommended that you calibrate your probe at least once a day before starting the sampling process. If you do not monitor dissolved oxygen levels frequently, you can calibrate the probe occasionally. More important data also requires more frequent calibrations. Calibration of the sensor is very important if accurate data is to be received.